Polímeros de impresión molecular para la liberación de antibióticos

Abstract

Se sintetizaron polímeros impresos molecularmente (MIP) utilizando ciprofloxacino con tres enfoques de polimerización. Se probaron dos monómeros funcionales, ácido láctico y ácido metacrílico y se sintetizaron polímeros sin plantilla (NIP). Se caracterizaron por microscopía electrónica de barrido, infrarrojo por transformada de Fourier. La isoterma de adsorción se determinó que existe mayor capacidad de adsorción del MIP con ácido metacrílico con el método de coprecipitación. Se obtuvo que los MIPs siguieron el modelo Korsmeyer-Peppas. Los MIPs se utilizaron para determinar la concentración mínima inhibitoria la cual se encontró de 0.031 a 0.016 μg L-1 para Staphylococcus aureus y de 0.004 a 0.031 μgL-1 para Escherichia coli. Finalmente, se evaluó la citotoxicidad en fibroblastos dérmicos, presentaron viabilidades superiores al 100%.

Molecularly imprinted polymers (MIPs) were synthesized using ciprofloxacin with three polymerization approaches. Two functional monomers, lactic acid and methacrylic acid, were tested and template-free polymers (NIP) were synthesized. They were characterized by Fourier transform infrared scanning electron microscopy. The adsorption isotherm was determined that there is a higher adsorption capacity of MIP with methacrylic acid with the coprecipitation method. It was obtained that the MIPs followed the model of Korsmeyer-Peppas. The MIPs were used to determine the minimum inhibitory concentration which was found to be from 0.031 to 0.016 μg L-1 for Staphylococcus aureus and from 0.004 to 0.031 μgL-1 for Escherichia coli. Finally, the cytotoxicity in dermal fibroblasts was evaluated, they presented viabilities greater than 100%.